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  • Title: Microtiterplate phosphate assay based on luminescence quenching of a terbium complex amenable to decay time detection.
    Author: Turel M, Duerkop A, Yegorova A, Karasyov A, Scripinets Y, Lobnik A.
    Journal: Anal Chim Acta; 2010 Aug 18; 675(1):42-8. PubMed ID: 20708114.
    Abstract:
    We describe a terbium-ligand complex (TbL) for a microtiterplate assay for phosphate (P) in the 0.3-100 micromol L(-1) range based on luminescence quenching. As the pH optimum is at neutral pH (7.4) the probe is quenched by both, primary (H(2)PO(4)(-)) and secondary phosphate (HPO(4)(2-)). The LOD is 110 nmol L(-1). A Stern-Volmer study revealed that quenching is mostly static. Due to the ms-decay time of TbL, the first luminescence lifetime assay for phosphate could also be developed. The lifetime-based calibration plot is linear between 0.5 and 5 micromol L(-1) of P. The effect of various surfactants on assay performance and a study on interferents are presented. The probe was successfully applied to determination of P in commercial plant fertilizers and validated against the molybdenum blue test. The probe is the most sensitive lanthanide-based probe for phosphate.
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