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  • Title: Strip-based amperometric detection of myeloperoxidase.
    Author: Windmiller JR, Chinnapareddy S, Santhosh P, Halámek J, Chuang MC, Bocharova V, Tseng TF, Chou TY, Katz E, Wang J.
    Journal: Biosens Bioelectron; 2010 Oct 15; 26(2):886-9. PubMed ID: 20708401.
    Abstract:
    The development of a screen-printed strip-based amperometric biosensor for the determination of myeloperoxidase (MPO) levels is reported. The biosensor utilizes 3,3',5,5'-tetramethylbenzidine (TMB) as a redox mediator to enable high-sensitivity quantification of physiological levels of MPO. A multivariate parameter optimization was performed. Under the optimal conditions, physiological levels of MPO between 3 and 18 U/L were detected in both acetate buffer (pH 4.5) and human serum using flexible screen-printed electrodes (SPE). The potential interference generated by common serum-based electroactive compounds and a similar peroxidase enzyme was also investigated. The proposed detection methodology offers a simpler, more rapid, and cost-effective alternative to conventional MPO immunoassays, thereby leading to further development in point-of-care testing of acute cardiac events.
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