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  • Title: [Correlation between ethambutol-resistance and embB gene mutation in Mycobacterium tuberculosis].
    Author: Li W, Xue X, Chu Y, Qiu Y, Song J, Zheng J.
    Journal: Wei Sheng Yan Jiu; 2010 Jul; 39(4):506-8. PubMed ID: 20726250.
    Abstract:
    OBJECTIVE: To analyze the characteristics of embB gene mutation in ethambutol-resistant Mycobacterium tuberculosis (M. TB) strains isolated from patients in Xi'an for establishing a rapid method to detect the mutation of embB gene in M. TB. METHODS: The embB gene of 104 Mycobacterium tuberculosis isolates was detected by PCR-Single Strand Conformation Polymorphism (PCR-SSCP) and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP). RESULTS: Taking strain H37Rv as a control, the SSCP and RFLP profiles of embB of 35 drug-sensitive strains in 104 Mycobacterium tuberculosis isolates were normal. The display of SSCP profiles in 39 of 69 (56.52%) ethambutol-resistant isolates and the display of RFLP profiles in 19 of 69 (27.54%) ethambutol-resistant isolates were abnormal. CONCLUSION: The EMB-resistance of M. TB strains isolated from patients in Xi'an is related to the mutation of embB gene, PCR-SSCP and PCR-RFLP are sensitive methods for detecting ethambutol-resistance of M. tuberculosis.
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