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  • Title: A laccase with antiproliferative activity against tumor cells from an edible mushroom, white common Agrocybe cylindracea.
    Author: Hu DD, Zhang RY, Zhang GQ, Wang HX, Ng TB.
    Journal: Phytomedicine; 2011 Mar 15; 18(5):374-9. PubMed ID: 20739163.
    Abstract:
    A laccase, with HIV-1 reverse transcriptase inhibitory activity (IC(50)=12.7 μM) and antiproliferative activity against HepG2 cells (IC(50)=5.6 μM) and MCF7 cells (IC(50)=6.5 μM), was purified from fresh fruiting bodies of the edible white common Agrocybe cylindracea mushroom. The laccase, which had a novel N-terminal sequence, displayed a molecular mass of 58 kDa within the range reported for most other mushroom laccases. The purification protocol entailed ion exchange chromatography on DEAE-cellulose, SP-Sepharose, and Q-Sepharose and gel filtration on Superdex 75. The laccase was adsorbed on DEAE-cellulose and Q-Sepharose, but unadsorbed on SP-Sepharose. Its optimum pH was pH 3-4 and its optimum temperature was 50°C. The activity of the isolated laccase differed from one substrate to another. The ranking was ABTS>N,N-dimethyl-1,4-phenylenediamine>hydroquinone>catechol>2-methylcatechol>pyrogallol.
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