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  • Title: [Effect of an abrupt rise in 2,4-dinitrophenol concentration on the growth of a turbidostat Saccharomyces cerevisiae culture].
    Author: Kaliuzhin VA.
    Journal: Mikrobiologiia; 1990; 59(5):756-63. PubMed ID: 2074851.
    Abstract:
    The purpose of the work was to study the effect of an abrupt rise in 2,4-dinitrophenol concentration on the growth of a turbidostat Saccharomyces cerevisiae 14 culture. The action of DNP caused three types of response. The growth of S. cerevisiae 14 ceased when DNP concentration was raised once to 10 mg per litre at 30 degrees C and pH 4, but did not stop if that was done in a gradual manner. It took at least 0.25 to 0.5 h for the culture to develop a protection against the action of DNP at a concentration of 10 mg per litre, and a minimal DNP concentration inducing the protection was from 0.6 to 0.9 mg per litre. The pH factor could also induce DNP protection. The dynamics of changes in DNP concentration was followed up in the cells in the course of transient processes. Apparently, the ability of yeast cells to grow in media with DNP is determined by the inhibitor concentration within the first 1.5 h. Apparently, adaptation of the culture to DNP is realised due to changes in the properties of the cell membrane complex.
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