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  • Title: Genetic manipulation of Mycobacterium abscessus.
    Author: Medjahed H, Singh AK.
    Journal: Curr Protoc Microbiol; 2010 Aug; Chapter 10():Unit 10D.2. PubMed ID: 20812214.
    Abstract:
    This unit covers genetic manipulation protocols for M. abscessus, including nucleic acid extraction (plasmid DNA, genomic DNA, and RNA), transformation, and a recombineering mutagenesis method. M. abscessus is a Biosafety Level 2 (BSL-2) bacterium, and working considerations are also discussed.
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