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  • Title: Use of metal chelate affinity chromatography for removal of zinc ions from alkaline phosphatase from Escherichia coli.
    Author: Lubińska VK, Muszyńska G.
    Journal: J Chromatogr; 1990 Nov 28; 522():171-7. PubMed ID: 2081752.
    Abstract:
    Alkaline phosphatase from Escherichia coli (APEC) is not retained at 4 degrees C on a metal-free tris(carboxymethyl)ethylenediamine (TED) column, but at 15 degrees C the metalloenzyme becomes bound to the gel. Chromatography of phosphatase on metal-free TED gel indicates a decline in its enzymic activity and zinc content to about 26% and 40%, respectively. The activity of chromatographed APEC can be partially restored by addition of zinc ions, indicating that metal-free TED gel is capable of removing zinc ions from alkaline phosphatase.
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