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  • Title: Efficient and cost-effective extraction of genomic DNA from formalin-fixed and paraffin-embedded tissues.
    Author: Weiss AT, Delcour NM, Meyer A, Klopfleisch R.
    Journal: Vet Pathol; 2011 Jul; 48(4):834-8. PubMed ID: 20817894.
    Abstract:
    Diagnostic and investigative molecular pathology frequently has to resort to extraction of DNA from formalin-fixed and paraffin-embedded tissue samples. Although many different protocols are reported for this type of material, extraction of sufficient amounts of intact DNA is still challenging. Here, the authors report a reproducible, simple, cost-effective, and efficient protocol that yields up to 140 μg of DNA from approximately 10 to 15 mg of formalin-fixed and paraffin-embedded tissue samples and compare it to available protocols. The protocol allows stable amplification of DNA fragments up to 600 bp in length in a wide variety of tissues.
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