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  • Title: Enzyme immunoassay of testosterone using nitrocellulose discs as the solid phase.
    Author: Samanta AK, Ali E.
    Journal: J Clin Chem Clin Biochem; 1990 Dec; 28(12):943-7. PubMed ID: 2081966.
    Abstract:
    An enzyme immunoassay (EIA) for the measurement of serum testosterone has been developed using nitrocellulose paper discs as the solid support. The paper discs (6 mm diameter) coated with testosterone-specific antibody were incubated with testosterone and testosterone-peroxidase conjugate in glass tubes. The amount of testosterone present in samples could be estimated from the bound peroxidase activity. The assay was validated by comparison with a microtitre plate-based ELISA and a commercial radioimmunoassay (RIA) kit. The correlation coefficient between RIA and EIA was 0.84. No significant cross reactivity was observed with other steroids, except for dihydrotestosterone. The inter- and intra-assay coefficients of variation were 4.4 and 9.6% respectively. The preservation and transport of the coated paper discs are convenient and the overall cost of the method is less than that of other methods.
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