These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.
    Author: Ito K, Ito S, Shimamura T, Kawarasaki Y, Abe K, Misaka T, Kobayashi T, Iwata S.
    Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun; 2010 Sep 01; 66(Pt 9):1086-8. PubMed ID: 20823533.
    Abstract:
    Glucansucrases encoded by Streptococcus mutans play essential roles in the synthesis of sticky dental plaques. Based on amino-acid sequence similarity, glucansucrases are classified as members of glycoside hydrolase family 70 (GH 70). Data on the crystal structure of GH 70 glucansucrases have yet to be reported. Here, the GH 70 glucansucrase GTF-SI from S. mutans was overexpressed in Escherichia coli strain BL21 (DE3), purified to homogeneity and crystallized using the hanging-drop vapour-diffusion method. Orthorhombic GTF-SI crystals belonging to space group P2(1)2(1)2 were obtained. A diffraction data set was collected to 2.1 A resolution.
    [Abstract] [Full Text] [Related] [New Search]