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  • Title: Whole fruit staining with aniline blue at harvest is associated with superficial pathogenesis of "Fuji" apples after storage.
    Author: Li F, Zhang X, Yao Y, Sun X, Liu L.
    Journal: Biotech Histochem; 2011 Dec; 86(6):394-403. PubMed ID: 20854224.
    Abstract:
    Whole "Fuji" apples (Malus domestica Borkh cv. Fuji) were treated with 0.2% aniline blue before storage in 2006, 2007 and 2008 to determine whether cuticular microcracking was associated with post-storage disorders. After storage for 7 months at 0° C and 90% relative humidity followed by 3 days at 20° C, a higher aniline blue staining scale value was associated with a higher peel browning and decay index. These results indicate that superficial disorders or diseases of apples may be related to cuticular microcracking that can be seen by aniline blue staining. Scanning electron microscopy was used to analyze the ultrastructure of stained portions of the cuticular complex. Disorders or diseases of the cuticle of epidermal tissue was associated with cracked lenticels, unhealed microcracks around the edge of the lenticel, and collapsed epicuticular wax; these areas stained more intensely. Our results indicated the potential of using an aniline blue staining prior to storing the fruit to predict the ultimate quality.
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