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  • Title: [Optimization of lyophilization procedures for freeze-drying of human red blood cells].
    Author: Chen LF, Liu JH, Wang DQ, Ouyang XL, Zhuang Y, Che J, Yu Y, Li H.
    Journal: Nan Fang Yi Ke Da Xue Xue Bao; 2010 Sep; 30(9):2055-8. PubMed ID: 20855248.
    Abstract:
    OBJECTIVE: To investigate the different parameters of the lyophilization procedures that affect the recovery of the rehydrated red blood cells (RBCs). METHODS: Human RBCs loaded in tubes were cooled with 4 different modes and subjected to water bath at 25 degrees celsius;. The morphological changes of the RBCs were observed to assess the degree of vitrification, and the specimens were placed in the freeze-dryer with the temperature set up at 40, -50, -60, -70 and -80 degrees celsius;. The rates of temperature rise of the main and secondary drying in the lyophilization procedures were compared, and the water residue in the specimens was determined. RESULTS: The protectant did not show ice crystal in the course of freezing and thawing. No significant difference was found in the recovery rate of the rehydrated RBCs freeze-dried at the minimum temperature of -70 degrees celsius; and -80 degrees celsius; (P > 0.05). The E procedure resulted in the maximum recovery of the RBCs (83.14% ± 9.55%) and Hb (85.33% ± 11.42%), showing significant differences from the other groups(P < 0.01 or 0.05). The recovery of the RBCs showed a positive correlation to the water residue in the samples. CONCLUSION: Fast cooling in liquid nitrogen and shelf precooling at -70 degrees celsius; with a moderate rate of temperature rise in lyophylization and a start dry temperature close to the shelf equilibrium temperature produce optimal freeze-drying result of human RBCs.
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