These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Technical note: Bovine acute-phase response after corticotrophin-release hormone challenge.
    Author: Cooke RF, Bohnert DW.
    Journal: J Anim Sci; 2011 Jan; 89(1):252-7. PubMed ID: 20870951.
    Abstract:
    The objective of this study was to evaluate plasma concentrations of cortisol, acute-phase proteins, and proinflammatory cytokines in beef steers after an intravenous corticotrophin-release hormone (CRH) infusion. Six weaned, halter-trained Angus steers (BW = 163 ± 7.0 kg; age = 203 ± 5.8 d) were fitted with indwelling jugular catheters on d -1 of the study and assigned to receive intravenously 0.1 μg of bovine CRH/kg of BW on d 0 of the study. Blood samples were collected every hour via jugular catheters from -1 to 8 h, and every 6 h via jugular venipuncture from 12 to 72 h relative to CRH infusion (0 h). Steer rectal temperature was assessed concurrently with each blood collection. Samples collected from -1 to 8 h relative to CRH infusion were analyzed for plasma concentrations of IL-1β and IL-6, tumor necrosis factor-α, interferon-γ, cortisol, ceruloplasmin, and haptoglobin, whereas samples collected from 12 to 72 h were analyzed for plasma concentrations of ceruloplasmin and haptoglobin only. Plasma cortisol concentrations were greater (P < 0.01) at 1, 2, and 3 h compared with prechallenge values (2.8, 2.3, 13.9, 8.2, and 5.7 ng/mL for -1, 0, 1, 2, and 3 h, respectively; SEM = 0.6). Mean postchallenge IL-6 concentrations were greater (P = 0.04) compared with mean prechallenge values (1.49 vs. 1.34 log pg/mL; SEM = 0.05), whereas IL-6 concentrations at 6 h were greater (P ≤ 0.05) compared with prechallenge values (1.36, 1.33, and 1.61 log pg/mL at -1, 0, and 6 h, respectively; SEM = 0.09). Rectal temperatures were greater (P < 0.01) at 2 and 8 h compared with prechallenge values (38.9, 39.0, 39.3, and 39.5°C at -1, 0, 2, and 8 h, respectively; SEM = 0.07). Plasma ceruloplasmin concentrations were greater (P = 0.03) at 54 h (29.6, 31.5, and 35.2 mg/dL at -1, 0, and 54 h, respectively; SEM = 1.7), whereas plasma haptoglobin concentrations were greater (P < 0.05) at 54, 66, and 72 h compared with prechallenge values (3.3, 2.8, 4.3, 4.6, and 4.1 absorbance at 450 nm × 100 at -1, 0, 54, 66, and 72 h, respectively; SEM = 0.24). In conclusion, intravenous CRH infusion at 0.1 μg/kg of BW increased circulating concentrations of cortisol and stimulated the acute-phase response in halter-trained beef steers.
    [Abstract] [Full Text] [Related] [New Search]