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  • Title: Determination of pethidine in human plasma by LC-MS/MS.
    Author: Wang X, Xiang Z, Cai X, Wu H, Wang X, Li J, Zhang M.
    Journal: Biomed Chromatogr; 2011 Jul; 25(7):833-7. PubMed ID: 20872940.
    Abstract:
    A sensitive and selective liquid chromatography-tandem mass spectrometry method for the determination of pethidine in human plasma was developed and validated over the concentration range of 4-2000 ng/mL. After addition of ketamine as internal standard, liquid-liquid extraction was used to produce a protein-free extract. Chromatographic separation was achieved on a 100 × 2.1 mm, 5 µm particle, Allure™ PFP propyl column, with 45:40:15 (v/v/v) acetonitrile-methanol-water containing 0.2% formic acid as mobile phase. The MS data acquisition was accomplished by multiple reactions monitoring mode with positive electrospray ionization interface. The lower limit of quantification was 4 ng/mL; for inter-day and intra-day tests, the precision (RSD) for the entire validation was less than 7%, and the accuracy was within 95.9-106.5%. The method is sensitive and simple, and was successfully applied to analysis of samples of clinical intoxication.
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