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  • Title: Phosphatidylinositol 4,5-bisphosphate-induced conformational change of ezrin and formation of ezrin oligomers.
    Author: Carvalho K, Khalifat N, Maniti O, Nicolas C, Arold S, Picart C, Ramos L.
    Journal: Biochemistry; 2010 Nov 02; 49(43):9318-27. PubMed ID: 20873751.
    Abstract:
    The plasma membrane-cytoskeleton interface is a dynamic structure involved in a variety of cellular events. Ezrin, a protein from the ERM family, provides a direct linkage between the cytoskeleton and the membrane via its interaction with phosphatidylinositol 4,5-bisphosphate (PIP₂). In this paper, we investigate the interaction between PIP₂ and ezrin in vitro using PIP₂ dispersed in a unimolecular way in buffer. We compared the results obtained with full-length ezrin to those obtained with an ezrin mutant, which was previously found not to be localized at the cell membrane, and with the N-terminal membrane binding domain (FERM domain) of ezrin. We show that PIP₂ induced a conformational change in full-length ezrin. PIP₂ was also found to induce, in vitro, the formation of oligomers of wild-type ezrin, but not of mutant ezrin. These oligomers had previously been observed in vivo, but their role is yet to be clarified. Our finding hints at a possible role for PIP₂ in the formation of ezrin oligomers.
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