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  • Title: Fast analysis of isoflavones by high-performance liquid chromatography using a column packed with fused-core particles.
    Author: Manchón N, D'Arrigo M, García-Lafuente A, Guillamón E, Villares A, Ramos A, Martínez JA, Rostagno MA.
    Journal: Talanta; 2010 Oct 15; 82(5):1986-94. PubMed ID: 20875606.
    Abstract:
    The recent development of fused-core technology in HPLC columns is enabling faster and highly efficient separations. This technology was evaluated for the development of an fast analysis method for the most relevant soy isoflavones. A step-by-step strategy was used to optimize temperature (25-50°C), flow rate (1.2-2.7 mL/min), mobile phase composition and equilibration time (1-5 min). Optimized conditions provided a method for the separation of all isoflavones in less than 5.8 min and total analysis time (sample-to-sample) of 11.5 min. Evaluation of chromatographic performance revealed excellent reproducibility, resolution, selectivity, peak symmetry and low limits of detection and quantification levels. The use of a fused-core column allows highly efficient, sensitive, accurate and reproducible determination of isoflavones with an outstanding sample throughout and resolution. The developed method was validated with different soy samples with a total isoflavone concentration ranging from 1941.53 to 2460.84 μg g(-1) with the predominant isoflavones being isoflavone glucosides and malonyl derivatives.
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