These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Stability of plasma adrenocorticotrophic hormone (ACTH): influence of hemolysis, rapid chilling, time, and the addition of a maleimide.
    Author: Livesey JH, Dolamore B.
    Journal: Clin Biochem; 2010 Dec; 43(18):1478-80. PubMed ID: 20875812.
    Abstract:
    OBJECTIVES: The aim of this study was to examine the effects of hemolysis, rapid chilling, time, and the addition of a maleimide on the stability of human plasma ACTH measurements. DESIGN AND METHODS: Partially hemolyzed EDTA blood (n=10), initially at 37°C, was centrifuged at 4°C either immediately or after rapid chilling in ice/water. Plasma ACTH was then measured either immediately, or after 1h at 22°C with or without the addition of 2mM N-phenyl maleimide (NPM). RESULTS: For 0.2% hemolysis compared to no hemolysis, the mean (±SEM) loss with immediate centrifugation and immediate ACTH measurement was 11±1%. This loss was significantly (p<0.002) reduced to 6±1% by an initial rapid chilling of the samples. For analysis after 1h at 22°C, the addition of NPM decreased the loss of ACTH from 15±2% to 2±2% (p<0.002). CONCLUSION: Rapid chilling, prompt analysis, and addition of NPM can each reduce the interference of hemolysis in the measurement of plasma ACTH concentrations.
    [Abstract] [Full Text] [Related] [New Search]