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  • Title: Monocarboxylate carrier from bovine heart mitochondria: reconstitution in a functionally active state.
    Author: Nałecz KA, Zambrowicz BE.
    Journal: Acta Biochim Pol; 1990; 37(1):109-12. PubMed ID: 2087900.
    Abstract:
    A method has been developed for functional reconstitution of the isolated pyruvate carrier. Optimal conditions were reached after removal of deoxycholate with 27 mg Triton X-100 per gram of moist Amberlite XAD-2 beads and 7 micrograms protein/mg of phospholipid. Uptake of pyruvate into proteoliposomes and sensitivity of the carrier towards inhibitors resemble those in intact mitochondria.
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