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  • Title: Immunological characterization of L-triiodothyronine and L-thyroxine labelled with rhodamine B isothiocyanate.
    Author: Reyes A, Diéguez JL, Ruiz M, Alonso A, Morell M.
    Journal: Rev Esp Fisiol; 1990 Sep; 46(3):269-72. PubMed ID: 2091151.
    Abstract:
    In the present work the label of L-T3 and L-T4 with RBITC have been developed by means of a relatively easy procedure. A purification by TLC, previously optimized, offers the possibility of obtaining a good recovery of conjugates T3 and T4-RBITC. The immunoreactivity of conjugates was calculated by a formulas especially designed for this purpose. This formulas could be applied to any molecule labelled with fluorescent compounds.
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