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  • Title: Histones H1 and H4 are present near the replication fork.
    Author: Stefanovsky V, Dimitrov S, Russanova V, Pashev I.
    Journal: Mol Biol Rep; 1990 Nov; 14(4):231-5. PubMed ID: 2094801.
    Abstract:
    The presence of histones H1 and H4 at the sites of actual DNA synthesis has been studied with Ehrlich ascites tumour cells, pulse labeled for different times with 3H-thymidine and then treated with formaldehyde to crosslink histones to DNA. The fixed chromatin fragments were sonicated to reduce the size of DNA, purified in a CsCl gradient and immunoprecipitated with antibodies to histones H1 and H4. Determination of specific radioactivity in precipitated probes showed that both histones have been associated with nascent DNA even upon 1 min pulse with 3H-thymidine, thus indicating their presence near the replication fork.
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