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  • Title: Screening and identification of a fungal β-glucosidase and the enzymatic synthesis of gentiooligosaccharide.
    Author: Qin Y, Zhang Y, He H, Zhu J, Chen G, Li W, Liang Z.
    Journal: Appl Biochem Biotechnol; 2011 Apr; 163(8):1012-9. PubMed ID: 20963514.
    Abstract:
    After screening with 0.1% esculoside and 0.03% FeCl(3), we identified from rotten wood a fungal isolate HML0366 that produces high amount of β-glucosidase. Phenotypic and rDNA internal transcribed spacer sequence analyses indicated that the isolate belongs to Aspergillus oryzae. The β-glucosidase produced by HML0366 had an activity of 128 U/g. high performance liquid chromatography analysis also demonstrated a high transglycosylation activity of the crude enzyme. The β-glucosidase was stable between pH 4-10 at 60 °C. A gentiobiose yield of 30.86 g/L was achieved within 72 h of the enzymatic reaction at pH 5 and 55 °C using 50% glucose as the substrate. For the first time, we report here the isolation of an A. oryzae strain producing β-glucosidase with high hydrolytic activities. The crude enzyme has a high transglycosylation activity, which enables the enzymatic synthesis of gentiooligosaccharides.
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