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  • Title: [Kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase].
    Author: Lebedeva OV, Ugarova NN, Berezin IV.
    Journal: Biokhimiia; 1977 Aug; 42(8):1372-9. PubMed ID: 20989.
    Abstract:
    A kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase within the pH range of 3.7-9.0 has been carried out. It was shown that the reaction of o-dianisidine peroxidase oxidation obeys the Michaelis--Menten kinetics; the kcat and Km values within the pH range used were determined. The optimum of peroxidase catalytic activity during o-dianisidine oxidation was observed at pH 5.0-6.0. The kinetic pattern of the reaction is discussed. It was demonstrated that deprotonation of the group at pK 6.5 decreases the kcat value 60 times. At pH greater than 8.0 an additional ionogenic group controls the enzyme activity.
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