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  • Title: Separation and some properties of an endo-1,4-beta-D-xylanase from Aspergillus flavipes.
    Author: Sherief AA.
    Journal: Acta Microbiol Hung; 1990; 37(3):301-6. PubMed ID: 2100903.
    Abstract:
    A fungal strain isolated from stored wheat straw and identified as Aspergillus flavipes was found to produce an extracellular endo-1,4,beta-D-xylanase when grown in shake-culture of a mineral salt medium containing 0.1% wheat strawxylan as a sole source of carbon for 3 days at 28 degrees C. The enzyme was separated by precipitation with ammonium sulphate and desalted by Sephadex G-25 column. Fractionation and purification of the enzyme were carried out by chromatography on Bio-Gel P-100. The purified enzyme (mol wt 45,000) was found to release xylooligomers only from beta-1,4-xylan. It showed maximum activity at pH 5.0 and temperature 55 degrees C.
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