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  • Title: [Fermentation of two insoluble dietary fibers by colonic microflora in vitro].
    Author: Peng X, Pang Q, Wu X, Zhang N.
    Journal: Wei Sheng Yan Jiu; 2010 Sep; 39(5):611-4. PubMed ID: 21033443.
    Abstract:
    OBJECTIVE: To investigate the rule of insoluble dietary fiber fermentation in intestinal tract. METHODS: Using destarched wheat bran and sugarcane residue as substrates for the fermentation of colonic microflora in vitro in anaerobic conditions. Samples were collected at 0, 4, 8, 12 and 24 h after fermentation, and the pH value, selective plate colony counts were measured and the content of SCFAs were detected with GC. RESULTS: After fermenting for pH 5.43. The change of colony counts of Bifidobacterium, Lactobacillus, Enterococci, Enterobacter, Bacteroides and total anaerobic bacterium was not significant in two media with different substrates at different fermentation periods. Both destarched wheat bran and sugarcane residue could be utilized by colon microbials to produce SCFAs, most of which was propionate at the amount of 0.58 mg/ml and 0.56 mg/ml respectively. During the fermentation process, lactic acid was accumulated and promptly consumed by colonic microflora. CONCLUSION: Insoluble dietary fiber could be fermented by intestinal microflora. The growth succession of colonic microflora might be varied during fermentation.
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