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  • Title: [Quantitative determination of forsythiaside in Forsythia suspensa].
    Author: Xia B, Zhu J, Wang Z, Lin L, Gao H.
    Journal: Zhongguo Zhong Yao Za Zhi; 2010 Aug; 35(16):2110-2. PubMed ID: 21046741.
    Abstract:
    OBJECTIVE: To develop a quantitative determination of Forsythia suspensa and to provide scientific basis for its quality control. METHOD: By HPLC, the separation was performed on a Zorebax Eclipse XDB-C18 column (4.6 mm x 250 mm, 5 microm) at 25 degrees C using a isocratic elution of acetonitrile-water (containing 0.4% glacial acetic acid, 15:85). The detection wavelength was 330 nm. RESULT: The calibration curve showed a good linearity (r = 0.9998) within test range of 0.202-1.515 microg. And the average recovery was 98.54% with RSD 1.1%. The content range of forsythoside A in 11 batches of Forsythiae Fructus was 0.200%-1.681%. CONCLUSION: The developed method is simple, accurate, specific, and with good repeatability. It is suitable for quality evaluation of Forsythiae Fructus.
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