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  • Title: Analysis of the rat JE gene promoter identifies an AP-1 binding site essential for basal expression but not for TPA induction.
    Author: Timmers HT, Pronk GJ, Bos JL, van der Eb AJ.
    Journal: Nucleic Acids Res; 1990 Jan 11; 18(1):23-34. PubMed ID: 2106664.
    Abstract:
    We have cloned the immediate-early serum-reponsive JE gene from the rat in order to study the regulation of this gene. We show that sequences of the JE promoter region confer serum-inducibility to a reporter gene. Analysis of the promoter in transient assays reveals that: i) the -141/-88 region is required for the response to the phorbol ester TPA, ii) the -70/-38 region is essential for basal activity. This latter region harbors the sequence TGACTCC, which resembles the consensus site for AP-1 binding, TGACTCA. DNA-protein binding assays indicate that the JE AP-1 site and the consensus AP-1 site have an overlapping but not identical binding spectrum for AP-1 proteins. Our data suggest that the inability of some AP-1 sites to respond to TPA is caused by subtle differences in affinity for AP-1 proteins.
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