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  • Title: The K+-dependent phosphatase from human renal tissue: its properties and time dependent inhibition by ouabain.
    Author: Braughler JM, Corder CN.
    Journal: Arch Int Pharmacodyn Ther; 1978 Jun; 233(2):192-9. PubMed ID: 210729.
    Abstract:
    The K+-dependent p-nitrophenyl phosphatase activity associated with human renal (Na+ + K+)-ATPase was examined for some of its kinetic properties. ATP inhibited the K+-dependent phosphatase and raised the Km for p-nitrophenylphosphate. Stimulation of the K+-dependent phosphatase by K+ was blocked by Na+ in a noncompetitive manner. Inhibition of the K+-dependent phosphatase by ouabain was dependent upon incubation time. The apparent Kï was 2.0 micron.
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