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  • Title: Transmembrane potential measurement with carbocyanine dye diS-C3-(5): fast fluorescence decay studies.
    Author: Síp M, Herman P, Plásek J, Hrouda V.
    Journal: J Photochem Photobiol B; 1990 Jan; 4(3):321-8. PubMed ID: 2107293.
    Abstract:
    The mechanism of carbocyanine dye diS-C3-(5) fluorescence intensity variations with transmembrane potential changes has been studied using time-resolved fluorescence spectroscopy. Clear evidence is given of the transmembrane-potential-dependent partition of the dye among various sites with different fluorescence lifetimes. It was found that fluorescence decay profiles reflect the transmembrane potential changes.
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