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  • Title: Isolation of oligotrophic denitrifiers carrying previously uncharacterized functional gene sequences.
    Author: Ishii S, Ashida N, Otsuka S, Senoo K.
    Journal: Appl Environ Microbiol; 2011 Jan; 77(1):338-42. PubMed ID: 21075882.
    Abstract:
    Oligotrophic denitrifying bacteria, including those belonging to the genera Herbaspirillum, Azospirillum, and Bradyrhizobium, were obtained using a single-cell isolation technique. The taxonomic composition of the denitrifier population was similar to those assessed by previous culture-independent studies. The sequencing of nitrite reductase and N(2)O reductase genes of these strains revealed previously unknown links between 16S rRNA and the denitrification-functional gene phylogenies. In particular, we identified Bradyrhizobium strains that harbor nirS sequences previously detected only in culture-independent studies.
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