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  • Title: Utilization of DBS within drug discovery: development of a serial microsampling pharmacokinetic study in mice.
    Author: Clark GT, Haynes JJ, Bayliss MA, Burrows L.
    Journal: Bioanalysis; 2010 Aug; 2(8):1477-88. PubMed ID: 21083348.
    Abstract:
    BACKGROUND: Dried blood spots (DBS) are rapidly gaining a foothold in the pharmaceutical industry. However, applications in exploratory drug discovery are limited mainly owing to method development time. The development of a generic DBS assay is presented with its application in serial microsampling from mice. RESULTS: A generic 'fit-for-purpose' assay was developed on FTA(®) Elute, which allowed 90% of compounds tested to reach sensitivity levels of 1 ng/ml. A ten-time point serial mouse pharmacokinetic study was conducted using 20-µl microsamples and DBS. Application of generic 'fit-for-purpose' approach did not compromise data delivery or quality. CONCLUSIONS: Serial microsampling and DBS in exploratory mouse pharmacokinetic has been shown to provide superior data quality when compared with traditional plasma-based composite studies.
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