These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Effects of PTEN over-expression on phosphatidyl inositol 3-kinase/protein kinase B signal pathway in ovarian epithelial cancer cells].
    Author: Zhai YN, Xu LL, Shen Y, Xia H, Shen YF.
    Journal: Zhonghua Fu Chan Ke Za Zhi; 2010 Sep; 45(9):682-5. PubMed ID: 21092549.
    Abstract:
    OBJECTIVE: To evaluate the effect of exogenous wild PTEN gene stable transfected into human ovarian cancer cell line HO-8910 on phosphatidyl inositol 3-kinase (PI3K)/protein kinase B (Akt) signal pathway and cells proliferation. METHODS: Wild-type PTEN recombinant eukaryotic expression plasmid was constructed and then was transfected into HO-8910 cells by lipofectamine 2000. The expression of PTEN, Akt1, Akt2, PI3K mRNA and protein of PTEN were tested by reverse transcription (RT)-PCR and Western blot. The proliferation of HO-8910 after wild PTEN gene transfected was measured by methyl thiazolyl tetrazolium (MTT). RESULTS: Wild-type PTEN gene was successfully transfected into HO-8910 cells. The results of RT-PCR and western bolt showed that there were the significant expression high level of PTEN mRNA and protein after infected by wild-PTEN plasmid than those in the control [(17,372 ± 23) vs. (39 ± 1) vs. (78 ± 4) copies/ml, P < 0.05]. While the expression of mRNA of Akt1, Akt2 and PI3K were decreased clearly than those in the control [(28 ± 2) vs. (115 ± 5), (7 ± 1) vs. (18 ± 2), (61 ± 2) vs. (84 ± 2) copies/ml, all P < 0.05]. The proliferation rate of HO-8910 cells was obviously slower than those in the control (90 158 ± 47 vs. 148,251 ± 65 vs. 250,115 ± 62, P < 0.05). CONCLUSION: Transfection of PTEN may increase the expression of PTEN and inhibit the proliferation of HO-8910 cells, in which PI3K/Akt signal pathway is inhibit significantly.
    [Abstract] [Full Text] [Related] [New Search]