These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: An automated assay of glycogen phosphorylase in the direction of phosphorolysis.
    Author: Schreiber WE, Bowling S.
    Journal: Ann Clin Biochem; 1990 Mar; 27 ( Pt 2)():129-32. PubMed ID: 2109566.
    Abstract:
    We have developed a rapid, automated assay for glycogen phosphorylase that measures the degradative reaction. The substrate contains higher concentrations of phosphate and AMP than other methods, and the enzyme is preincubated with the substrate before adding phosphate to start the reaction. These modifications improve the activity and linearity of the assay. The new assay compares favourably with a standard phosphorylase assay in the synthetic direction and is linear to 500 U/L. We have used this assay to measure phosphorylase activity in human tissue samples and muscle biopsy specimens.
    [Abstract] [Full Text] [Related] [New Search]