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  • Title: Molecular cloning and differential expression patterns of copper/zinc superoxide dismutase and manganese superoxide dismutase in Hypophthalmichthys molitrix.
    Author: Zhang ZW, Li Z, Liang HW, Li L, Luo XZ, Zou GW.
    Journal: Fish Shellfish Immunol; 2011 Feb; 30(2):473-9. PubMed ID: 21112403.
    Abstract:
    Copper/zinc superoxide dismutase (Cu,Zn-SOD) cDNA and manganese superoxide dismutase (Mn-SOD) cDNA were first cloned from silver carp Hypophthalmichthys molitrix using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) method. The open reading frame (ORF) of Cu,Zn-SOD is 465 bp and encodes a 154 amino acids (aa) protein, whereas the ORF of Mn-SOD is 675 bp and encodes a 224 aa protein. Multiple polypeptide sequence alignment showed high identity both of Cu,Zn-SOD (70-87%) and Mn-SOD (80-96%) with the species compared. Both Cu,Zn-SOD and Mn-SOD were detected in heart, brain, liver, kidney, spleen, muscle, gill and blood. Cu,Zn-SOD and Mn-SOD were expressed throughout the embryogenesis, indicating their important roles during embryonic development specially at the cleavage stage. Acute hypoxia suppressed expression of Cu,Zn-SOD and Mn-SOD in liver significantly, up-regulated them in gill relatively, indicating that tissue-specific expression of Cu,Zn-SOD and Mn-SOD is an important stress response adapted to hypoxia.
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