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  • Title: Efficient expression and Zn(II)-dependent structure of the DNA binding domain of the yeast GAL4 protein.
    Author: Serikawa Y, Shirakawa M, Matsuo H, Kyogoku Y.
    Journal: Protein Eng; 1990 Mar; 3(4):267-72. PubMed ID: 2111549.
    Abstract:
    Three protein fragments of different sizes which contain the DNA binding domain of transcription factor GAL4 from Saccharomyces cerevisiae have been expressed in functional forms in Escherichia coli. DNase I footprinting and gel retardation assays showed that the purified proteins bound to the same DNA sequence on the gal1-gal10 promoter as intact GAL4 does. Denaturation--refolding experiments demonstrated that Zn(II) is necessary for maintenance of the conformation of the DNA binding domain of GAL4, as judged on UV-CD and 1H-NMR measurements, as well as for specific DNA binding.
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