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  • Title: [Deoxyribonuclease from Corynebacterium diphtheriae: dynamics of synthesis and properties].
    Author: Iusupova DV, Bening GP, Sokolova RB, Fedorov RV.
    Journal: Vopr Med Khim; 1990; 36(1):27-31. PubMed ID: 2111600.
    Abstract:
    Diphtheritic bacteria of PW-8 Massachusetts strain produced into cultural medium only one nucleotidase--endoDNAase. The enzyme was synthesized by the cells during the exponential phase of growth. The DNAase was purified 500-fold and exhibited properties specific to neutral-alkaline DNAases (pH optimum about 7.5, absolute requirements for Me2+, single-step mechanism of substrate hydrolysis). The following properties were typical for the enzyme: absence of distinct specificity to structure of bases surrounding the hydrolyzed bond, formation of 5'-end phosphate groups and slightly higher preference to denatured DNA.
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