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  • Title: Biochemical properties of free and immobilized Candida rugosa lipase onto Al2O3: a comparative study.
    Author: Yeşiloğlu Y, Şit L.
    Journal: Artif Cells Blood Substit Immobil Biotechnol; 2011 Aug; 39(4):247-51. PubMed ID: 21117873.
    Abstract:
    Lipase from Candida rugosa (CRL) was immobilized by physical adsorption onto Al(2)O(3). The immobilization yield was more than 95% during 30 min. The properties of free and immobilized enzyme were also searched and compared. The optimum pH was shifted from 7 to 8.5 by immobilization of the enzyme. The maximum activity of the free and the immobilized enzymes occured at 35 and 40°C, respectively.
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