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  • Title: Oligonucleotide-stabilized fluorescent silver nanoclusters for sensitive detection of biothiols in biological fluids.
    Author: Han B, Wang E.
    Journal: Biosens Bioelectron; 2011 Jan 15; 26(5):2585-9. PubMed ID: 21123043.
    Abstract:
    In this work, we reported a simple and sensitive method to detect biothiols, such as cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), using fluorescent silver nanoclusters (Ag NCs) stabilized by single-stranded DNA (DNA-Ag NCs) as probes. The photoluminescence intensity of DNA-Ag NCs was found to be quenched effectively with the increase of biothiols concentration due to the formed nonfluorescent coordination complex between DNA-Ag NCs and biothiols, resulting in the shift-to-red of emission wavelength. But the fluorescence of DNA-Ag NCs was not changed in the presence of other amino acids at 10-fold higher concentration. Satisfactory detection limits and linear relationships of Cys, GSH and Hcy were obtained, respectively. The resulted plots exhibited good linear relationships in the range from 8.0×10(-9) to 1.0×10(-7) mol L(-1) (R(2)=0.984) for Cys, 8.0×10(-9) to 1.0×10(-7) mol L(-1) (R(2)=0.983) for GSH, and 2.0×10(-6) to 6.0×10(-7) mol L(-1) (R(2)=0.999) for Hcy, respectively; the detection limits of Cys, GSH and Hcy were 4.0 nmol L(-1), 4.0 nmol L(-1), and 0.2 μmol L(-1), respectively. The method was successfully used for the detection of biothiols in human plasma samples.
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