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  • Title: Bovine dental pulp collagens: characterization of types III and V collagen.
    Author: Tsuzaki M, Yamauchi M, Mechanic GL.
    Journal: Arch Oral Biol; 1990; 35(3):195-200. PubMed ID: 2112377.
    Abstract:
    Pulp was essentially solubilized by partial pepsin digestion. The various genetic types of collagens were isolated by differential salt precipitation and extraction. Types I, III and V collagen represented 56, 41 and 2% of the total collagen, respectively. The type V collagen comprised two different molecular species consisting of [alpha 1(V)]2 alpha 2(V) and alpha 1(V) alpha 2(V) alpha 3(V), the ratio of which was approx. 1:1.3. The major portion of the type III collagen was present as a high molecular weight aggregate which released alpha 1(III) chains upon reduction with 2-mercaptoethanol.
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