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  • Title: Inhibition of gene function in mammalian cells using short-hairpin RNA (shRNA).
    Author: Henriksen JR, Buechner J, Løkke C, Flægstad T, Einvik C.
    Journal: Methods Mol Biol; 2011; 703():189-204. PubMed ID: 21125491.
    Abstract:
    RNAi is now the preferred method for silencing gene expression in a variety of systems. In this chapter we describe the procedure for applying short-hairpin RNA (shRNA) to study gene function. Detailed descriptions of target site selection, shRNA construction, shRNA transfection and target knockdown validation are included.
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