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  • Title: Presence of free viral DNA in simian virus 40-transformed nonproducer cells.
    Author: Daya-Grosjean L, Monier R.
    Journal: J Virol; 1978 Aug; 27(2):307-12. PubMed ID: 211262.
    Abstract:
    Extracts from several simian virus 40 (SV40)-transformed nonproducer cells were prepared by the hot-phenol procedure normally used to extract cellular RNA. These extracts contained SV40 infectious units. Part of the infectious units were identified as SV40 form I DNA molecules. The results of reconstruction experiments suggest that SV40 form I DNA is extractable by the hot-phenol procedure because of its fast renaturation rate. The significance of the presence of free viral DNA in nonproducer transformed cells is discussed.
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