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  • Title: Development of an enzyme-linked immunosorbent assay to detect serum antibody to chicken anemia agent.
    Author: Todd D, Mackie DP, Mawhinney KA, Connor TJ, McNeilly F, McNulty MS.
    Journal: Avian Dis; 1990; 34(2):359-63. PubMed ID: 2114871.
    Abstract:
    An enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to chicken anemia agent (CAA) has been developed. This test utilizes a CAA-specific mouse monoclonal antibody to selectively capture virus antigen. Chicken antibodies to CAA bind to the captured antigen and are detected with horseradish peroxidase-labeled anti-chicken immunoglobulin using a conventional indirect ELISA protocol. When 388 chicken sera from specific-pathogen-free and commercial flocks from the United Kingdom, West Germany, the United States and Australia were examined, 98.5% agreement was obtained between the results of the ELISA and the indirect immunofluorescence assay. This ELISA should have worldwide application in testing SPF and commercial chicken flocks for CAA antibodies.
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