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  • Title: Identification of new minor metabolites of penicillin G in human serum by multiple-stage tandem mass spectrometry.
    Author: Ho HP, Lee RJ, Chen CY, Wang SR, Li ZG, Lee MR.
    Journal: Rapid Commun Mass Spectrom; 2011 Jan 15; 25(1):25-32. PubMed ID: 21154651.
    Abstract:
    Liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) were applied to characterize drug metabolites. Although these two methods have overcome the identification and structural characterization of metabolites analysis, they remain time-consuming processes. In this study, a novel multiple-stage tandem mass spectrometric method (MS(n) ) was evaluated for identification and characterization of new minor metabolism profiling of penicillin G, one of the β-lactam antibiotics, in human serum. Seven minor metabolites including five phase I metabolites and two phase II metabolites of penicillin G were identified by using data-dependent LC/MS(n) screening in one chromatographic run. The accuracy masses of seven identified metabolites of penicillin G were also confirmed by mass spectral calibration software (MassWorks™). The proposed data-dependent LC/MS(n) method is a powerful tool to provide large amounts of the necessary structural information to characterize minor metabolite in metabolism profiling.
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