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  • Title: Analysis of a commercial dimethyl-sulfoxide-stabilized frozen canine platelet concentrate by turbidimetric aggregometry.
    Author: Guillaumin J, Jandrey KE, Norris JW, Tablin F.
    Journal: J Vet Emerg Crit Care (San Antonio); 2010 Dec; 20(6):571-7. PubMed ID: 21166978.
    Abstract:
    OBJECTIVES: To assess platelet function of a commercial dimethyl-sulfoxide (DMSO)-stabilized frozen platelet concentrate (PC) using turbidimetric aggregometry. DESIGN: In vitro analysis. SETTING: Research laboratory in a school of veterinary medicine. ANIMALS: Five units of frozen PC in 6% DMSO were studied. Fresh platelet-rich plasma (PRP), with and without 6% DMSO, from 6 healthy dogs were used as controls. INTERVENTIONS: Turbidimetric platelet aggregation was measured after initiation of platelet aggregation by addition of adenosine diphosphate (ADP), collagen, or thrombin at concentrations of 30 μM, 20μg/mL, and 0.5U/mL, respectively. Measures were performed at thaw and repeated 2 hours after thaw for the frozen PC. MEASUREMENTS AND MAIN RESULTS: Compared with PRP, the frozen PC showed decreased aggregation in response to thrombin (amplitude of 84% versus 25%, P=0.01), and collagen (amplitude of 13% versus 3%, P=0.05) but not ADP (6.5% versus 18%, P=0.2). Compared with frozen PC at thaw, the frozen PC at 2 hours after thaw showed decreased aggregation in response to thrombin, collagen, and ADP (P<0.05). There was no difference in aggregation between PRP in 6% DMSO and frozen PC. CONCLUSIONS: These in vitro data suggest there is a decrease in platelet response to agonists associated with the freeze-thaw process in the commercially available 6% DMSO canine frozen PC.
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