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  • Title: Highly sensitive electrochemiluminescent biosensor for adenosine based on structure-switching of aptamer.
    Author: Zhu X, Zhang Y, Yang W, Liu Q, Lin Z, Qiu B, Chen G.
    Journal: Anal Chim Acta; 2011 Jan 17; 684(1-2):121-5. PubMed ID: 21167993.
    Abstract:
    A highly sensitive and selective electrochemiluminescent (ECL) biosensor for the determination of adenosine was developed. Single DNA (capture DNA) was immobilized on the gold electrode through Au-thiol interaction at first. Another DNA modified with tris(2,2'-bipyridyl) ruthenium(II)-doped silica nanoparticles (Ru-SNPs) that contained adenosine aptamer was then modified on the electrode surface through hybridizing with the capture DNA. In the presence of adenosine, adenosine-aptamer complex is produced rather than aptamer-DNA duplex, resulting with the dissociation of Ru-SNPs-labeled aptamer from the electrode surface and the decrease in the ECL intensity. The decrease of ECL intensity has a direct relationship with the logarithm of adenosine concentration in the range of 1.0×10(-10) to 5.0×10(-6)molL(-1). The detection limit of the proposed method is 3.0×10(-11)molL(-1). The existence of guanosine, cytidine and uridine has little interference with adenosine detection, demonstrating that the developed biosensor owns a high selectivity to adenosine. In addition, the developed biosensor also demonstrates very good reusability, as after being reused for 30 times, its ECL signal still keeps 91% of its original state.
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