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  • Title: [Determination of virus concentration using a minicomputer].
    Author: Smola T, Specht G, Liebermann H.
    Journal: Arch Exp Veterinarmed; 1990; 44(2):311-7. PubMed ID: 2117430.
    Abstract:
    Described in this paper is a physical method by which to determine virus concentrations, including ultracentrifugation of a sample through a saccharose gradient and subsequent analysis of tubule content by means of a flow photometer. Here before, the curves thus obtained used to be graphically evaluated by means of stencil plates. Direct recording and processing of the extinction-time curve by means of a KC 85/1 minicomputer have substantially improved accuracy of results. Time consumption has been reduced, and reading as well as calculating errors have been entirely ruled out. Repetition of determinations confirmed that virus concentrations could be determined at an accuracy of four to seven percent.
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