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  • Title: Cryo-electron tomography: methodology, developments and biological applications.
    Author: Vanhecke D, Asano S, Kochovski Z, Fernandez-Busnadiego R, Schrod N, Baumeister W, Lučić V.
    Journal: J Microsc; 2011 Jun; 242(3):221-7. PubMed ID: 21175615.
    Abstract:
    Cryo-electron tomography allows three-dimensional visualization of frozen-hydrated, vitrified biological material at molecular resolution. Here, we summarize the most important sample preparation methods and technical aspects relevant for cryo-electron tomography, as well as its recent biological applications from isolated macromolecular complexes to entire cells and tissues.
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