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  • Title: Epitope analysis of peanut allergen Ara h1 with human monoclonal IgM antibody clone #86.
    Author: Shinmoto H, Takeda M, Matsuo Y, Naganawa Y, Tomita S, Takano-Ishikawa Y.
    Journal: Hum Antibodies; 2010; 19(4):101-5. PubMed ID: 21178281.
    Abstract:
    A human-mouse hybridoma clone #86 secreting IgM-class human monoclonal antibody to peanut allergen protein Ara h1 was newly established. To detect an antibody-binding sequence (epitope) on Ara h1, the monoclonal antibody #86 was reacted with multi-pin apparatus with a series of peptides synthesized from the amino acid sequence of Ara h1. The antibody #86 was found to bind to a peptide with amino acid sequence of 481EEEEDEDEEEEGSNREVRRY500. Further analysis with shorter pin-peptides with ten amino acid-long showed that the peptides reacted with the antibody #86 contained a sequence of 485DEDEEEE491. This might be an essential linear sequence of this epitope. When the 485DED487 part of the peptide was replaced by alanine, decreased binding of antibody #86 was observed.
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