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  • Title: Wnt inhibitors enhance chondrogenesis of human mesenchymal stem cells in a long-term pellet culture.
    Author: Im GI, Lee JM, Kim HJ.
    Journal: Biotechnol Lett; 2011 May; 33(5):1061-8. PubMed ID: 21210183.
    Abstract:
    The long-term effects (~3 weeks) of two Wnt inhibitors (dickkopf [DKK]-1 and secreted frizzled-related protein [sFRP]-1), on the chondrogenic differentiation of human mesenchymal stem cells (hMSCs) was determined. Wnt inhibitors significantly increased the amount of glycosaminoglycan (GAG) in treated pellets (P<0.05). The gene expression of COL2A1 increased and COL1A1 decreased while the gene expression of SOX-9 and COL10A1 did not change significantly after three weeks of in vitro culture. The protein expression of type II collagen significantly increased (P<0.05) and that of type I collagen significantly decreased (P<0.05) while SOX-9 and type X collagen protein expression was unaffected. These findings suggest that Wnt inhibitors promote the chondrogenic differentiation of hMSCs when treated for three weeks.
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