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  • Title: [Cloning and sequencing of immunoglobin lambda-chain cDNA in american mink (Mustela vison].
    Author: Naiakshin AM, Belousov ES, Taranin AV, Bogachev SS, Rogozin IB, Baranov OK.
    Journal: Genetika; 1990 Aug; 26(8):1527-31. PubMed ID: 2124200.
    Abstract:
    cDNA library in the lambda gt11 phage was constructed using poly (A+)-mRNA from mink spleen as a template. Immunoscreening of the library allowed the identification of 2 lambda-related clones containing 370 and 803 bp insertion (lambda IGL-1 and lambda IGL-2). Analysis of the primary structure of lambda IGL-2 demonstrated that it contains a large portion of V lambda-segment, J lambda-segment, C lambda-gene and its 3'-untranslated part. The nucleotide sequences known for the immunoglobulin genes were compared to the sequence of the lambda IGL-2 clone. The highest degree of homology was established for the rabbit lambda-genes, this being 63, 94 and 72% for the RF3 region of V lambda-segment, J lambda-segment and C lambda-region, respectively.
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