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  • Title: Microwave-assisted extraction and in situ clean-up for the determination of fluoroquinolone antibiotics in chicken breast muscle by LC-MS/MS.
    Author: Xu H, Chen L, Sun L, Sun X, Du X, Wang J, Wang T, Zeng Q, Wang H, Xu Y, Zhang X, Ding L.
    Journal: J Sep Sci; 2011 Jan; 34(2):142-9. PubMed ID: 21246719.
    Abstract:
    A new method was developed for the determination of six fluoroquinolone antibiotics including fleroxacin, levofloxacin, ciprofloxacin, lomfloxacin, enrofloxacin, and sparfloxacin in chicken breast muscle, in which the extraction and clean-up were performed in one step by microwave irradiation. The mixture of ACN containing 0.3% v/v phosphoric acid/water pH 3 (70:30, v/v) was used as the extraction solution and hexane was used as the clean-up solution. The extract was analyzed by liquid chromatography-tandem mass spectrometry system. The RSDs of intra- and inter-day obtained are in the range of 1.0-10.4 and 3.8-13.6%, respectively. In the three fortified levels of chicken breast muscle (20, 100, and 500 ng/g), the recoveries of fluoroquinolone antibiotics ranging from 66.0 to 97.2% are obtained. The LODs are in the range of 2.7-6.7 ng/g. This method simplifies the process of the sample preparation and reduces the operation errors.
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